人体常见细胞的免疫组化特性

人体常见细胞的免疫组化特性

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时间：2003-7-9 11:55:36 来源：北京协和医院病理科 阅读2449次   Lymph Node

 

Germinal center B cells

 

CD45+; CD20+; CD79a+ (weak); CD5-; CD10+; Ig+ (IgG, IgM or IgA, but not IgD); Light chain+; bcl-2-; bcl-6+; proliferating cases in dark zone well highlighted by Ki67

 

Mantle zone B cells:

 

CD45+; CD20+; CD79a+; IgD+; IgM+; IgG-; IgA-; Light chain+; bcl-2+; CD5-; CD10-; bcl-6-

 

Marginal zone B cells and monocytoid B cells

 

CD45+; CD20+; CD79a+; IgM+; IgD-/+; CD5-; bcl-2-; bcl-6-

 

Interfollicular B cells

 

CD45+; CD20+; CD79a+; Ig+ (IgD, IgM, IgG or IgA); Light chain+; bcl-2+; bcl-6-; activated B cells may express CD30; EBV-infected B cell may upregulate CD43

 

Plasma cells

 

CD45-/+; CD20-; CD79a+/-; Ig+; CD138+; EMA+/-

 

T cells

 

CD3+; CD45RO+; CD43+; mostly TCR-??, sometimes TCR-??; CD4+ or CD8+; subpopulation can express cytolytic molecules (granzyme B, TIA1, perforin)

 

T cells in germinal center

 

CD3+; CD45RO+; CD43+; CD57+; CD4+;CD8-

 

NK cells:

 

CD45+; surface CD3-; cytoplasmic CD3+; CD43+; CD45RO+; CD56+; CD57+/-; Granzyme B+; TIA1+; Perforin+

 

Follicular dendritic cells

 

CD45-; CD21+; CD35+; FDC+; CD23 (subpopulation)+; S100-/+; CD1a-; lysozyme-; HLA-DR+

 

Interdigitating dendritic cells

 

CD45+; S100+; CD68-/+; CD1a-/+; lysozyme-/+; HLA-DR+; CD43+

 

Langerhans cells

 

CD45+; S100+; CD68-/+; CD1a+; lysozyme-/+; HLA-DR+

 

Histiocytes

 

CD45+; S100-; CD68+; CD1a-; lysozyme+; HLA-DR+; CD43+

 

Spleen Lymphocytes

 

Either B cells or T cells (see Lymph node)

 

Splenic sinus lining cells

 

Factor VIII+; CD31-/+; CD34-/+; CD8+; lysozyme+; CD68-/+

 

Splenic pulp cords

 

Rich in CD68+ cells

 

Thymus

 

Thymic epithelial cells

 

CK+ (esp. CAM5.2+, but 34?E12 shows up the epithelium of the medulla much better)

 

Thymic T lymphocytes in cortex

 

CD45+; CD3+; TdT+; CD1a+; CD99+; high Ki67 index

 

Thymic T lymphocytes in medulla

 

CD45+; CD3+; TdT-; CD1a-; CD99-

 

Thymic B lymphocytes in medulla

 

CD45+; CD20+ (sometimes with asteroid appearance); CD79a+

 

Skin and mucosal tissues and parenchymal organs

 

Epithelium

 

CK+ (glandular epithelium tends to express low molecular weight CK; and some epithelial cells may express specific CK subtypes); vimentin-/+; BerEP4+/-; EMA+/-; E-cadherin+/-

 

Special types of epithelium

 

* Skin epidermis

 

- 34?E12+, CAM5.2-, CK5/6+

 

* Prostatic acini and ducts

 

 -- PSA+

 

* Breast with apocrine change

 

 -- Brst2+

 

* Glandular epithelium

 

-- CEA may be positive (luminal); EMA often positive (esp. luminal)

 

* Pneumocytes

 

 -- Surfactant/PE10+, TTF1+

 

* Liver cells

 

-- CK+; HEP-PAR1+; CAM5.2+; AE1/AE3-; polyclonal CEA or CD10 in canalicular pattern; albumin+

 

* Bile ducts

 

 -- CK+;CAM5.2+; AE1/AE3+, HEP-PAR1-

 

Myoepithelium

 

CK+; CK14+; High molecular weight CK+; Actin+; calponin+; S100-/+; GFAP-/+; Vimentin+

 

Basal cells of prostate

 

CK+; CK14+; Actin-; S100-

 

Melanocytes

 

S100+; Vimentin+; HMB45- (some junctional melanocytes can be +); CK-; c-kit+

 

Merkel cells

 

CK+; CK20+; synaptophysin+; chromogranin+; NSE+; neurofilament+

 

Mesenchymal cells

 

Fibroblasts

 

Vimentin+; Actin-; Desmin-; CK-

 

Myofibroblasts

 

Vimentin+; Actin+; Desmin-/+; CK-/+

 

Smooth muscle

 

Vimentin+; Actin+; Desmin+; CK-/+

 

Interstitial dendritic cells

 

Vimentin+; CD34+; Actin-/+; Desmin-

 

Endothelium

 

Factor VIII related antigen+; CD31+; CD34+; Vimentin+

 

Pericytes

 

Factor VIII related antigen-; CD31-; CD34-; vimentin+; Actin+; Desmin-

 

Fat cells

 

Vimentin+; S100+

 

Cartilage cells

 

Vimentin+; S100+; GFAP+/-

 

Skeletal muscle

 

Vimentin+; Actin+/-; Desmin+; Myoglobin+; MyoD1- (positive only in primitive or fetal muscle cells); Myogenin- (positive only in primitive or fetal muscle cells); S100-/+ (weak staining); CD56-/+ (positive in damaged muscle)

 

Endometrial stromal cells

 

CK-; Vimentin+; Actin+/-; Desmin-/+; S100-; CD10+

 

Mesothelial cells

 

Mesothelial cells

 

CK+; CK5/6+; Vimentin+; Calretinin+; HBME1+; N-cadherin+; E-cadherin-; CEA-; BerEP4-; LeuM1-; Desmin -/+ (occasional)

 

Neuroendocrine and endocrine organsThyroid follicular cells

 

CK+; Thyroglobulin+; TTF1+; Chromogranin-; Synaptophysin-; NSE-

 

Thyroid C cells

 

CK+; Calcitonin+; TTF1+; Chromogranin+; Synaptophysin+; NSE+; CEA+

 

Parathyroid

 

CK+; Parathyroid hormone+; TTF1-; Chromogranin+;Synaptophysin+; NSE+; Neurofilament+

 

Islet cells

 

CK+; Synaptophysin+; Chromogranin+; NSE+; Various hormones+

 

Pituitary cells

 

CK+; Synaptophysin+; Chromogranin+; NSE+; Various hormones+

 

Adrenal cortical cells

 

CK+/-; Synaptophysin-; Chromogranin-; NSE-; melan-A+/-; inhibin+/-

 

Dispersed neuroendocrine cells

 

CK+; Synaptophysin+; Chromogranin+; NSE+; Various hormones+

 

Central and peripheral nervous system

 

Neurons and ganglion cells

 

CK-; Neurofilament+; NSE+; Synaptophysin+; Chromogranin-/+; Calretinin+/-; GFAP-

 

Nerve fibers (CNS or peripheral)

 

CK-; Neurofilament+; NSE+; Synaptophysin+; Chromogranin-/+; GFAP-; Leu7+

 

Astrocytes

 

CK-, GFAP+; S100+; Vimentin+; Synaptophysin-; Neurofilament-

 

Oligodendroglial cells

 

 CK-, GFAP-; S100+; Leu7+/-; Synaptophysin-; Neurofilament-

 

Ependymal cells

 

CK-/+; GFAP+; Vimentin+; EMA+/-

 

Choroid plexus cells

 

CK+; Transthyretin+; GFAP+/-; BerEP4-; CEA-; S100+/-

 

Schwann cells

 

CK-, S100+; GFAP-/+; Vimentin+; Neurofilament-; EMA-

 

Perineurial cells and meningeal cells

 

CK-, S100-; GFAP-; EMA+; Vimentin+

 

Paraganglion chief cells and adrenal medulla cells

 

CK-; Synaptophysin+; Chromogranin+; NSE+; Neurofilament+; S100-

 

Sustentacular cells

 

CK-, S100+; GFAP-/+; Synaptophysin-; Neurofilament+

 

Gonads

 

Sertoli cells

 

CK+/-; CD99+; Inhibin+; CD56-/+

 

Leydig cells

 

CK-; Synaptophysin-; CD56-/+; Inhibin+; Melan A+/-

 

Granulosa cells

 

CK-; Vimentin+; CD99+; Inhibin+; Melan A+/-

 

Germ cell (spermatogonia, ovum)

 

CK-; PLAP-; c-kit+

 

Placenta

 

Syncytiotrophoblast

 

CK+; HCG+

 

Cytotrophoblast

 

CK+; HCG-

 

Intermediate trophoblast

 

CK+; HCG-/+; HPL+

 

Decidual cells

 

CK-; Vimentin+

 

Bone marrow

 

Granulocytic series cells

 

CD45+; Myeloperoxidase+; KP1/CD68+; PGM1/CD68-; lysozyme+

 

Monocytic series cells

 

CD45+; Myeloperoxidase-; KP1/CD68+; PGM1/CD68+; lysozyme+

 

Red cell series

 

Glycophorin A+

 

Megakaryocytes and platelets

 

Factor VIII-related antigen+; CD31+; CD34+